Brain sections from rats microinjected with b-galactosidase (b-gal) protein using hemagglutinating virus of Japan envelope (HVJ-E) vector. Note the strong b-gal activity (A). X-Gal-stained neurons are seen at the site of microinjection (B). Arrows point to X-Gal-stained neuronal cells. CC, central canal. Scale bars, 100?m.
To the best of our knowledge, this paper is the first report of direct in vivo protein transduction into a specific restricted brain area.

(Owada-Makabe, K., Tsubota, Y., Yukawa, K., Kakimoto, N., Liang, X.-M., Ichinose, M., Maeda, M.: Direct in vivo protein transduction into a specific restricted brain area in rats. Neuroscience Letters, 378 (1): 18-21, 2005.)

SELECTED PUBLICATIONS IN ENGLISH (2000 ~ 2007)

1. Maeda, M., Waki, H., Kohsaka, A., Yukawa, K., Nakamura, T.: The Sympathoexcitatory Pathway from the CVL to the RVL for Controlling Brain Vessels. Tzu Chi Ned J, 2008, in press
2. Waki, H., Yamasaki, M., Katahira, K., Katsuda, S., Maeda, M., Shimizu, T.: Developmental changes in functional characteristics of aortic baroreceptor afferents in rats. Experimental Physiology, 93 (3): 319-324, 2008.
3. Maeda, M.: Chapter 11 Regulation of local blood flows by the cardiovascular center and new strategy for central regulation of circulation. In Kubo, T., Kuwaki, T. (eds.) Central mechanisms of cardiovascular regulation. Transworld Research Network, India, 2007, pp. 173-193. (Invited paper, Review book)
4. Duale H, Waki H, Howorth P, Kasparov S, Teschemacher AG, Paton JFR: Restraining influence of A2 neurons in chronic control of arterial pressure in spontaneously hypertensive rats. Cardiovasc Res 76: 184-193, 2007
5. Paton JFR, Waki H, Abdala AP, Dickinson J, Kasparov S: Vascular-brain signaling in hypertension: role of angiotensin II and nitric oxide. Curr Hypertens Rep 9: 242-247, 2007
6. Waki H, Kasparov S, Liu B, Miyake M, Katahira K, Murphy D, Paton JFR: Junctional adhesion molecule-1 is up regulated in the spontaneously hypertensive rat: evidence for a pro-hypertensive role within the brainstem. Hypertension 49: 1-7, 2007
7. St-John WM, Waki H, Dutschmann M, Paton JFR: Maintenance of eupnea of in situ and in vivo rats following riluzole: a blocker of persistent sodium channels. Respir Physiol Neurobiol. 15, 155(1):97-100, 2007
8. Tanaka T, Yukawa K, Umesaki N: Radiation reduces bleomycin sensitivity in cervical squamous cancer cells in vitro. Eur J Gynaec Oncol XXVIII, n.4: 273-277, 2007
9. Tanaka T, Yukawa K, Umesaki N: Radiation reduces carboplatin sensitivity and enhances nedaplatin sensitivity in cervical squamous cell carcinoma in vitro. Eur J Gynaec Oncol XXVIII, n.5: 352-355, 2007
10. Kohsaka A, Laposky A, Moynihan Ramsey K, Estrada C, Joshu C, Kobayashi Y, Turek FW, Bass J: High Fat Diet Disrupts Behavioral and Molecular Circadian Rhythms in Mice.
    Cell Metabolism 6: 414-21, 2007
11. McClurg P, Janes J, Wu C, Delano DL, Walker JR, Batalov S, Takahashi JS, Shimomura K, Kohsaka A, Bass J, Wiltshire T, Su AI: Genome-wide Association Analysis in Diverse Inbred Mice: Power and Population Structure. Genetics 176: 675-83, 2007
12. Gouraud SS, Heesom K, Yao ST, Qiu J, Paton JFR, Murphy D: Dehydration-induced proteome changes in the rat hypothalamo-neurohypophyseal system. Endocrinology. 148 (7):3041-52, 2007
13. Gouraud SS, Yao ST, Heesom KJ, Paton JFR, Murphy D: 14-3-3 proteins within the hypothalamic-neurohypophyseal system of the osmotically stressed rat: transcriptomic and proteomic studies. J Neuroendocrinol. 19 (11) : 913-22, 2007
14. Waki H, Paton JFR: Response to Junctional Adhesion Molecule-1 May Have a Wider Role in Cardiovascular Disease. Hypertension 50:e23,doi:10.1161/HYPERTENSIONAHA.107.093195, 2007
15. Kohsaka A , Bass J: A Sense of Time: How Molecular Clocks Organize Metabolism.
    Trends Endocrinol Metab 18:4-11, 2007
16. Moynihan Ramsey K, Marcheva B, Kohsaka A, Bass J: The Clockwork of Metabolism.
    Annu Rev Nutr 27:219-40, 2007
17. Yukawa K, Tanaka T, Bai T, Li L, Tsubota Y, Owada-Makabe K, Maeda M, Hoshino K, Akira S, Iso H: Deletion of the kinase domain from death-associated protein kinase enhances spatial memory in mice.
    Int J Mol Med 17(5):869-73, 2006
18. Tanaka T, Yukawa K, Umesaki N: Effective chemoradiotherapy protocol with 5-fluorouracil for cervical squamous cell carcinoma in vitro.
    Eur J Gynaec Oncol 2006 XXVII, n. 3: 243-246, 2006
19. Tanaka T, Bai T, Yukawa K, Umesaki N: Optimal combination chemotherapy and chemoradiotherapy with etoposide for advanced cervical squamous cancer cells in vitro.
    Oncol Rep 15(4):939-47,2006
20. Bai T, Tanaka T, Yukawa K, Umesaki N, Matsumoto M, Akira S: Impaired postnatal development in C/EBPβ-deficient mice.
    The Journal of Reproduction and Developement, 52(5): 645-9, 2006.
21. Bai T, Tanaka T, Yukawa K, Umesaki N: A novel mechanism for acquired cisplatin-resistance: Suppressed translation of death-associated protein kinase mRNA is insensitive to 5-aza-2'-deoxycitidine and trichostatin in cisplatin-resitant cervical squamous cancer cells.
    Int J Oncol 28(2):497-508, 2006
22. Waki H, Kasparov S, Murphy D, Paton JF: Endothelial nitric oxide synthase in nucleus tractus solitarii contributes to hypertension in spontaneously hypertensive rats.
    Hypertension 48: 644-650, 2006
23. Polson J, McCallion N, Waki H, Thorne G, Tooley M, Paton JF, Wolf A: Evidence for cardiovascular autonomic dysfunction in neonates withcoarctation of the aorta.
    Circulation, 113: 2844-2850, 2006
24. Waki H, Katahira K, Polson JW, Kasparov S, Murphy D, Paton JF: Automation of analysis of cardiovascular autonomic function from chronic measurements of arterial pressure in conscious rats. Exp Physiol 91: 201-213, 2006
25. Owada-Makabe K, Tsubota Y, Yukawa K, Kakimoto N, Ling X.-M, Ichinose M, Maeda M : Direct in vivo protein transduction into a specific restricted brain area in rats.
    Neurosci Lett 378(1) : 18-21, 2005
26. Tsubota Y, Owada-Makabe K, Yukawa K, Maeda M : Hypotensive effect of des-acyl ghrelin at uncleus tractus solitarii of rat.
    NeuroReport 16(2) : 163-6, 2005
27. Yukawa K, Kishino M, Hoshino K, Shirasawa N, Kimura A, Tsubota Y, Owada-Makabe K,
    Bai T, Tanaka T, Ueyama T, Ichinose M, Takeda K, Akira S, Maeda M : The kinase domain of death-associated protein kinase is inhibitory for tubulointerstitial fibrosis in chronic obstructive nephropathy.
    Int J Mol Med 15 (1) : 73-8, 2005.
28. Yukawa K, Kishino M, Goda M, Ling X.-M, Kimura A, Tanaka T, Bai T, Owada-Makabe K, Tsubota Y, Ueyama Y, Ichinose M, Maeda M, Takeda K, Akira S : STAT6 deficiency inhibits tubulointerstitial fibrosis in obstructive nephropathy.
    Int J Mol Med 15 (2 ) : 225-30, 2005.
29. Kumanogoh A, shikina T, Suzuki K, Uematsu S, Yukawa K, Kashiwamura S, Tsutsui H, Yamamoto M, Takamatu H, Ko-Mitamura EP, Takegahara N, Marukawa S, Ishida I, Morishita H, Prasad DV, Tamura M, Mizui M, Toyofuku T, Akira S, Takeda K, Okabe M, Kikutani H : Nonredundant roles of Sema4A in the immune system: defective T cell priming and TH1/TH2 regulation in Sema4A-deficient mice.
    Immunity 22(3) : 305-16, 2005
30. Yukawa K, Iso H, Tanaka T, Tsubota Y, Owada-Makabe K, Bai T, Takeda K, Akira S, Maeda M : Down-regulation of dopamine transporter and abnormal behavior in STAT6-deficient mice.
    Int J Mol Med 15(5) : 819-25, 2005
31. Yukawa K, Tanaka T, Bai T, Ueyama T, Owada-Makabe K, Tsubota Y, Maeda M, Suzuki K, Kikutani H, Kumanogoh A : Semaphorin 4A induces growth cone collapse of hippocampal neurons in a Rho/Rho-kinase-dependent manner.
    Int J Mol Med 16(1) : 115-8, 2005
32. Yukawa K, Hoshino K, Kishino M, Tsubota Y, Owada-Makabe K, Maeda M, Bai T, Tanaka T , Akira S : Deletion of the kinase domain from death-associated protein kinase attenuates p53 expression in chronic obstructive uropathy.
    Int j Mol Med 16(3) : 389-93, 2005
33. Tanaka T, Yukawa K, Umesaki N : Radiation enhances cisplatin-sensitivity in human cervical squamous cancer cells in vitro.
    Eur J Gynaecol Oncol 26(4) :431-3, 2005
34. Tanaka T, Bai T, Yukawa K, Utsunomiya T, Umesaki N : Reduced radiosensitivity and increased CD40 expression in cyclophosphamide-resistant subclones established from human cervical squamous cell carcinoma cells.
    Oncol Rep 14(4) :941-8, 2005
35. Tanaka T, Bai T, Yukawa K, Umesaki N : Radiation-induced cell death is independent of the apoptotic signals mediated by death-associated protein kinase in human cervical squamous cell carcinoma cells.
    Oncol Rep 14(4) :949-55, 2005
36. Yukawa K, Tanaka T, Owada-Makabe K, Tsubota Y, Bai T, Maeda M, Takeda K, Akira S, Iso H : Reduced prepulse inhibition of startle in STAT6-deficient mice.
    Int j Mol Med 16(4) : 673-5, 2005
37. Tanaka T, Toujima S, Utsunomiya T, Bai T, Utsunomiya H, Mori I, Yukawa K, Umesaki N :
    Cell proliferation of bulky cervical squamous cell carcinoma is strongly associated with a parecrine tissue-specific growth factor(s).
    Oncol Rep 14(5) :1121-6, 2005
38. Tanaka T, Yukawa K, Umesaki N : Combination effwcts of irradiation and irinotecan on cervical squamous cell carcinoma cells in vitro.
    Oncol Rep 14(5) : 1365-9, 2005
39. Kakimoto N : Hypotensiv effect of neuromedin U by microinjection into the rat cardiovascular-related region of the medulla oblongata.
    財団法人循環器学研究進行財団　平成16年度事業報告書 : 62-65, 2005
40. Bai T, Tanaka T, Yukawa K, Maeda M, Umesaki N : Reduced expression of death-associated protein kinase in human uterine and ovarian carcinoma cells.
    Oncol Rep 11 (3): 661-665, 2004
41. Yukawa K, Hoshino K, Kishino M, Mune M, Shirasawa N, Kimura A, Tsubota Y, Owada-Makabe K, Tanaka T, Ichinose M, Maeda M, Takeda K, Akira S : Deletion of the kinase domain in death-associated protein kinase attenuates renal tubular cell apoptosis in chronic obstructive uropathy.
    Int J Mol Med 13 (4): 515-520, 2004.
42. Yukawa K, Shirasawa N, Ohshima A, Mune M, Kimura A, Bai T, Tsubota Y, Owada-Makabe K, Tanak T, Kishino M, Tsuruo Y, Umesaki N, Maeda M : Localization of Death-associated protein kinase to renal tubule cells, and increased expression in rat chronic obstructive uropathy.
    J Nephrol 17 (1): 26-33, 2004.
43. Kishino M*, Yukawa K*, Hoshino K*, Kimura A, Shirasawa N, Otani H, Tanaka T, Owada-Makabe K, Tsubota Y, Maeda M, Ichinose M, Takeda K, Akira S, Mune M : (*equally contriduted) Deletion of the kinase domain in death-associated protein kinase attenuates tubular cell apoptosis in renal ischemia-reperfusion injury.
    J Am Soc Nephrol 15 (7): 1826-1834, 2004.
44. Tsubota Y, Miyashita E, Miyajima M, Owada-Makabe K, Yukawa K, Maeda M: The Wakayama Epileptic Rat (WER), a new mutant exhibiting tonic-clonic seizures and absence-like seizures. Exp Anim, 52 (1): 53-62, 2003.
45. Inoue M, Maeda M, Washio M, Harada N: Occupational health laws in Japan and various other countries: comparison for identification of specific shortcomings in Japan. Int Med J, 10 (1): 7-11, 2003.
46. Maeda M, Tsubota Y, Owada-Makabe K, Yukawa K, Kakimoto N: Regulation of local blood flow by the cardiovascular center and changes of circulattion by the microinjection of antisense oligonucleotide into the cardiovascular center of the medulla. Clin Exp Hypertens, 25 (5): 295-296, 2003.
47. Tsubota Y, Kakimoto N, Owada-Makabe K, Yukawa K, Liang X.-M, Mune M, Maeda M: Hypotensive effects of neuromedin U microinjected into the cardiovascular-related region of the rat uncleus tractus solitarius. NeuroReport, 114 (18): 2387-2390, 2003
48. Yamanashi K, Miyamae T, Sasaki Y, Maeda M, Hirano H, Misu Y, Goshima Y: Involvement of nitric oxide production via kynurenic acid-sensitive glutamate receptors in DOPA-induced depressor responses in the nucleus tractus solitarii of anesthetized rats. Neuroscience Research 43: 231-238, 2002
49. Nishino T, Kudo H, Doi Y, Meada M, Hamasaki K, Morita M, Fujimoto S : Immunocytochemistry of glutathione S-transferase in taste bud cells of rat circumvallate and foliate papillae. Chemical Senses, 26 (2): 179-188, 2001
50. Irie M, Meada M, Nagata S : Can conditioned histamine release occur under urethane anesthesia in guinea pigs ?
    Physiology & Behavior, 72 (4): 567-573, 2001
51. Murata H, Yamazaki S, Maeda M, Hisashige A : Input-output analysis of economic effect in nursing care insurance. Journal of Home Economics of Japan, 52 (8): 729-736, 2001
52. Inoue M, Minami Y, Masaki S, Kawashita T, Yoshioka K, Maeda M : Attitudes toward reports on general health examination results by age, judgement of findings and work-styles. Journal of Occupational Health, 43 (5): 281-283, 2001
53. Nakai M, Maeda M: Cerebral cortical muscarinic cholinergic and N-methyl-D-aspartate receptors mediate increase in cortical blood flow elicited by chemical stimulation of periaqueductal gray matter. Neuroscience 98 (3): 449-457, 2000
54. Washio M, Ishibashi N, Arai Y, Kiyohara C, Tanaka K, Maeda M, Fujishima M, Okayama M : The isolation of Methicillin-resistant Staphylococcus aureus (MRSA) is a predictor for poor prognosis of the elderly patients with bacterial infection. International Medical Journal 7 (2): 117-120, 2000
55. Yukawa K, Tanaka T, Toyomi A, Tsuji S, Akira S, Iso H: Impairedwater-maze performance in mice lacking trenscription factor DDAAT/enhancer-cription facter C/ATF by the cAMP signal activation in hippocampal neurons,binding protein β. Neuroscience research Communicatons 26:59-67, 2000
56. Shi W, Kumanogoh A, Watanabe C, Uchida J, Wang X, Yasui T, Yukawa K, Ikawa M, Okabe M, Parnes J R, Yoshida K, Kikutani H : The Class IVSemaphorin CD100 Plays Noruredant Roles in thelmmune S ystem: Defective B and T Cell Activationin CD100-Deficient Mice. Immunity 13: 633-642, 2000
57. Maeda M, Inoue M, Washio M : International comparison of periodic health examination and medical privacy of workers. Journal of UOEH 22 (Supplement): 165-168, 2000
58. Inoue M, Maeda M : Effect of different work styles on blood pressure. Journal of UOEH 22 (Supplement): 197-200, 2000
59. Maeda M : Physiological mechanisms of job stress. Lecture note on occupational health, 2000
    Published by Japan International Cooperation Agency and University of Occupational and Environmental Health, Kitakyushu, Japan. pp. 317-320, 2000